【摘要】 目的探讨胃癌细胞凋亡与其微环境中的免疫细胞凋亡和浸润程度的关系。方法应用免疫组织化学技术标记40例手术切除的胃腺癌组织微环境中的树突状细胞(DC)、T细胞和NK细胞,并对其浸润程度进行定量评价;应用TUNEL技术标记上述组织胃癌细胞与免疫细胞的凋亡细胞,并定量测出凋亡水平,最后分析胃癌细胞凋亡水平与免疫细胞的凋亡程度和不同免疫细胞浸润程度的关系。结果本组胃癌细胞的凋亡指数与其免疫细胞的凋亡指数呈显著负相关(r=-0.868,P<0.001),而与微环境中DC、T细胞和NK细胞的定量浸润水平呈显著正相关(r值分别为0.823、0.764和0.708,P值均<0.001)。结论胃癌细胞的凋亡水平与其微环境中的免疫细胞的浸润程度和凋亡水平密切相关。更多还原

【Abstract】 Objective To explore the possible relationship between the apoptosis of gastric carcinoma and its microenvironmental immunocytes.Methods The infiltrating degree(ID) of microenvironmental dentritic cells(DC),T cells and NK cells in 40 resected gastric adenocarcinoma(GC) specimens were evaluated quantitatively by immunohistochemistry.Their apoptotic index(AI) in the gastric GC and immunocytes was determined with TUNEL technique.The correlation between the AI of GC and the AI or ID of its microenvironmental immunocytes was analysed.Results The AI of GC was significantly negatively correlated with the AI of its microenvironmental immunocytes,but markedly positively correlated with the ID of its DC,T cells or NK cells(all P<0.001).Conclusion The apoptosis of gastric adenocarcinoma may be closely related to the apoptosis and infiltrating degree of its microenvironmental immunocytes.更多还原