【摘要】 目的:探讨缬沙坦对大鼠心肌梗死(MI)后心室重构和左室非梗死区(LVNIZ)心肌血管紧张素Ⅱ(AngⅡ)及醛固酮(Ald)的影响。方法:选取雌性Wistar大鼠结扎左冠状动脉,建立MI模型。术后将存活大鼠随机分为缬沙坦组给予缬沙坦15mg·kg-·1d-1直接灌胃给药,另设MI组、假手术组分别灌等量自来水,连续4周。心脏标本病理分析,测定左室截面直径(LVTD),VG染色测定LVNIZ的胶原容积分数(CVF),放免法测定LVNIZ的AngⅡ、Ald含量。结果:与假手术组比较,MI组LVTD(P<0.01)、CVF(P<0.01)、AngⅡ(P<0.05)及Ald(P<0.01)含量均显著增加;缬沙坦使增加的LVTD(P<0.05)、CVF(P<0.05)、AngⅡ(P<0.05)、Ald(P<0.01)显著降低。结论:缬沙坦可能通过抑制心肌局部AngⅡ及Ald的过表达而逆转心室重构。更多还原

【Abstract】 Objective:To investigate the effect of valsartan on ventricular remodeling and angiotensin Ⅱ and aldosterone in non-infarcted myocardium of left ventricle after myocardial infarction (MI). Methods:The rat model of MI was established by ligating the left coronary artery in female Wistar rats. Three hours after the ligation,21 survived rats were randomly divided to MI group,valsartan group,and sham operation group. The rats were lavaged with 15 mg·kg-1·d-1 valsartan in valsartan group and tap water in MI and sham operation groups for 4 weeks. The left ventricular transverse diameter (LVTD),which indicates the changes of left ventricular structure,was detected. The collagen volume fraction (CVF) in non-infarcted myocardium was detected after HE staining,and the contents of angiotensin Ⅱ and aldosterone in non-infarcted myocardium were determined with radioimmunoassay. Results:Compared with sham operation group,LVTD,CVF,and the contents of angiotensin Ⅱ and aldosterone were all significantly increased in MI group. LVTD,CVF,and the contents of angiotensin Ⅱ and aldosterone in valsartan group were all significantly lower than those in MI group. Conclusion:Valsartan may improve the ventricular remodeling after MI by inhibiting the overexpression of angiotensin Ⅱ and aldosterone.更多还原