节点文献
致绵羊脑炎型肠球菌溶血素CylA基因的克隆及序列测定
Cloning and analysis of DNA sequence of gene CylA of enterococci inducing sheeps Encephalitis
【摘要】 以致绵羊脑炎型肠球菌(Enterococcus)染色体DNA为模板,PCR扩增溶血素CylA的结构基因,将PCR产物克隆于pMD19-T载体,对PCR产物进行测序鉴定。与已发表的肠球菌溶血素CylA基因序列比较,同源性达99.3%。
【Abstract】 DNA fragment of CylA gene was amplified from the chromosomal DNA of Eerococci Inducing Sheeps Encephalitis by PCR and cloned into pMD19-T Vector and sequenced.DNA sequence comparison of the amplified fragment with the pub- lished sequence of gene CylA showed 99% nucleotide homology.
【关键词】 绵羊脑炎型肠球菌溶菌素；
CylA基因克隆；
测序；
【Key words】 eerococci inducing sheeps encephalitis； CylA cloning； sequencing；
【Key words】 eerococci inducing sheeps encephalitis； CylA cloning； sequencing；
- 【文献出处】 中国预防兽医学报 ,Chinese Journal of Preventive Veterinary Medicine , 编辑部邮箱 ,2007年04期
- 【分类号】S852.61
- 【被引频次】16
- 【下载频次】104