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猪圆环病毒间接免疫荧光方法的建立
The development of an indirect immunofluorescent technique of porcine circovirus
【摘要】 将接种PCV2且经多次传代的PK-15带毒细胞分散到96孔细胞培养板上,制成抗原诊断板,应用间接免疫荧光技术检测血清中PCV2的抗体。通过对各种反应物的工作浓度与作用时间的优化,建立了PCV2抗体的间接免疫荧光检测技术,可用于PCV2抗体水平的监测,为今后的猪圆环病毒疫苗抗体检测的研究提供了检测方法。
【Abstract】 An indirect immunofluorescent technique for the antibody detection (IFA) of porcine circovirus(PCV) was established using PK-15 cell on 96 cell plates. The optimization of the concentration and time was done. The technique has high sensitivity and specificity. The method is valuable to the research by providing the technique of detecting the antibody produced by the vaccine.
- 【文献出处】 中国预防兽医学报 ,Chinese Journal of Preventive Veterinary Medicine , 编辑部邮箱 ,2007年01期
- 【分类号】S852.5
- 【被引频次】66
- 【下载频次】1474