【摘要】 目的研究供体角膜消毒方法,观察抗生素和消毒剂两种处理技术的效果,预防角膜移植术后感染。方法用定性培养法和稀释法,对抗生素和消毒剂处理角膜的抗菌效果进行了检测。结果用1000单位的妥布霉素和含有效碘5000 mg/L聚维酮碘分别对供体角膜浸泡处理30 min和5 min,手术时对其保存液及剩余的角巩膜环分别进行细菌培养,妥布霉素处理组细菌检出阳性率为15.38%;聚维酮碘处理组细菌检出阳性率为5.13%。结论用有效碘5000 mg/L聚维酮碘或1000单位妥布霉素浸泡处理供体角膜,均显示出比较好的抗菌效果;聚维酮碘抗菌效果效果好于妥布霉素。更多还原

【Abstract】 Objective To study the method for disinfection of donor cornea and to observe the efficacy of two treatment techniques,i.e.using antibiotics and disinfectants,for prevention of infection after cornea transplantation.Method Qualitative culture method and dilution method were used to examine the anti-septic efficacy of the cornea treated with antibiotics or disinfectants.Results Donor corneas were immersed in 1000 U of tobramycin or povidone iodine containing available iodine 5000 mg/L for 30 min and 5 min respectively and the preserving solution and residual corneoscleral ring were cultured.The bacteria-positive rate of tobramycin treatment group was 15.38% and that of povidone iodine treatment group was 5.13%.Conclusion Treatment of donor cornea with immersion in povidone iodine containing available iodine 5000 mg/L or 1000 U of tobramycin showed relatively good antiseptic efficacy.The antiseptic efficacy of povidone iodine was better than that of tobramycin.更多还原