【摘要】 建立了一种用高效液相色谱法快速测定食醋中苯甲酸和山梨酸的新方法。以Zorbax SBC18(4.6mm×250mm×5μm)为分离柱,甲醇-0.05mol/LNaH2PO4溶液(磷酸调pH3.5)(35∶65)为流动相,流速1.0mL/min,波长230nm,进行检测,方法回收率在95.0%~105.3%之间,日内、日间R.S.D.在1.1~2.1之间,苯甲酸和山梨酸的检测限可达0.5μg/mL和0.75μg/mL,具有良好的准确度和再现性。更多还原

【Abstract】 A novel method is established for the determination of benzoic acid(BA) and sorbic acid(SA) in vinegar by high performance liquid chromatography(HPLC) rapidly.BA and SA was separated on a Zorbax SB C18(4.6mm×250mm×5μm).The mobile phase was methanol-0.05mol/LNaH2PO4 solution(pH adjusted to 3.5 with H3PO4),the flow-rate was 1.0 mL/min and the detective wavelength was 230 nm.The average recovery ranged from 95.0%～105.3%,and the R.S.D.of intra-day and inter-day assay were within 1.1～2.1,the detection limits of BA and SA were 0.5μg/mL and 0.75μg/mL.It is a simple and rapid method with good reproducibility.更多还原