【摘要】 用TTC法检测44例经过抗菌药物治疗的患子宫内膜炎荷斯坦奶牛。其中,41例采用子宫内灌注药物治疗,主要药物有土霉素、金霉素和庆大霉素;每个病例治疗1￣5次不等,3例采用静脉注射,主要药物有磺胺嘧啶钠和头孢噻啶。结果表明,31例经土霉素子宫内灌注的奶牛,停药后第24h所采的奶样均可检出抗生素;其中6头奶牛在第6d仍然抗生素阳性,残留时间最长的可达8d。8例用金霉素子宫灌注治疗的奶牛,停药后第48h所采的奶样,抗生素检出率100%,其在牛奶中的残留时间为2￣5d不等。2例经庆大霉素子宫灌注治疗的患病牛,药残时间分别为1￣2d。3头经静脉滴注治疗的病牛,牛奶中的药残时间约达到9d以上。此外,从3头用金霉素子宫内灌注的奶牛采集牛奶用于乳酸菌发酵试验。结果表明,牛奶TTC阳性时,发酵产酸较低。在治疗后6h采集的牛奶都不能正常发酵产酸、凝固。3例试验牛牛奶发酵产生的酸度分别于治疗后第13、24和48h恢复到治疗前的水平。更多还原

【Abstract】 The2,3,5-triphenyltetrazolium chloride(TTC) assay was used to investigate the drug’s residues in milk collected from 44 dairy cows with endometriris after antibacterial therapy.Of the 44 cases,41 were treated by 1 to 5 intrauterine infusions of oxytetracycline,chlortetracycline hydrochloride or gentamicin,and 3 were treated mainly by intravenous injections of sulfadiazine of cefaloridine.The results indicated that all 31 cases produced TTC positive milk 1 day after intrauterine infusions of oxytetracycline with 6 cases remaining TTC positive at 6 days after the last infusion.8 cases treated with intrauterine infusion of chlortetracycline produced TTC positive milk 2 days after treatment with a drug residue persisting in milk from 2 to 5 days.2 cases treated with gentamicin were detected TTC positive at 1 and 2 days,respectively.Milk residue persisting more than 9 days was found in the cases treated with intravenous injection of antibacterial drugs. In addition,milk samples were collected from 3 cows treated by intrauterine infusion of chlortetracycline hydrochloride, and then inoculated with a yogurt making bacterial culture to test their yogurt making ability.The results indicated that the milk samples showing TTC positive were not fermented normally;fermentation was badly inhibited in milk samples collected 6 hours after the treatment and recovered 13,24 or 48 hours later.更多还原