【摘要】 目的探讨对苯二酚与血清白蛋白的相互作用。方法在0.05 mol/L Na2HPO4-0.05 mol/L NaH2PO4-0.10 mol/L NaCl缓冲溶液中(pH=7.30),观察对苯二酚及牛血清白蛋白与对苯二酚结合后在L-半胱氨酸修饰金电极上的电化学行为。结果牛血清白蛋白与对苯二酚结合后可使对苯二酚的氧化峰峰电位正移,还原峰电位负移,且使苯二醌还原峰电流减小,根据对苯二酚、对苯二醌和血清白蛋白结合的饱和度,计算苯二酚、对苯二醌和血清白蛋白结合比分别为48:1和16:1。结论牛血清白蛋白与对苯二酚结合以较强的氢键结合,对苯二醌与血清白蛋白结合力较大。更多还原

【Abstract】 Objective To explore the interaction between p-hydroquinone and serum protein.Methods The cyclic voltammetry(CV) of p-hydroquinone and conjoint p-hydroquinone with serum protein at L-cysteine bodified golden electrode in 0.05 mol/L Na2HPO4-0.05 mol/L NaH2PO4-0.10 mol/L NaCl buffer solution(pH=7.30) was studied in this paper.Results The peak of conjoint hydroquinone with serum protein shifted to positive,the current of conjoint p-benzoquionone with serum protein decreased,and peak of conjoint p-benzoquionone with serum protein shifted to negative.According to the saturation of respective conjoint hydroquinone and p-benoquionone with serum protein,the conjoint rate of hydroquinone and p-benzoquionone with serum protein was 48:1 and 16:1 respectively.Conclusion The interaction between p-hydroquinone and serum protein ascribes to hydrogen bonding,however,this force between serum protein and p-benzoquionone is more than that between p-hydroquinone and serum protein.更多还原