【摘要】 目的在体外观察人肺腺癌细胞(SPC-A1)对谷胱甘肽(GSSG)修饰的纳米Fe3O4的内吞作用及与培养温度、时间及浓度的关系。方法通过透射电镜(TEM)与普鲁士兰染色法观察SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞作用。采用原子吸收光谱(AAS)法进行SPC-A1细胞粘附量及内吞量的定量分析。结果TEM结果表明,在4℃下培养24 h后GSSG修饰的纳米Fe3O4吸附于SPC-A1细胞表面;37℃下培养1 h后SPC-A 1细胞可内吞GSSG修饰的纳米Fe3O4细胞数目明显增加。AAS分析表明,SPC-A1细胞表面纳米Fe3O4的粘附量同培养时间及浓度呈正相关。GSSG修饰的纳米Fe3O4浓度较低(0.1或0.4 mg/ml)时,内吞量在24 h后达到饱和;GSSG修饰的纳米Fe3O4浓度较高(0.7或1.0 mg/ml)时,内吞量在72 h后达到饱和,单个细胞内吞量最大可达160 pg。结论SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞需要能量,内吞量依赖于浓度及培养时间。更多还原

【Abstract】 Objective To study the relationship between endocytosis of human lung adenocarcinoma cell line SPC-A1 modified by oxidized Glutathione（GSSG） and culture conditions including culture temperature,time and concentration.Methods The uptake of GSSG-modified Fe₃O₄ nanoparticles by SPC-A1 cell was observed by transmission electron microscopy（TEM） and Prussian blue staining.The extracellular adhesion or intracellular uptake of GSSG-modified Fe₃O₄ nanoparticles were measured by atom absorb spectrum（AAS）.Results TEM results indicated that GSSG-modified Fe₃O₄ nanoparticles adhered on the membrane of SPC-A1 cell after being cultured at 4?℃ for 1?h or 24?h,but SPC-Al cell could took up nanoparticles particles after cultured at 37?℃ for 1?h.Prussian blue staining indicated that SPC-Al cells containing GSSG-modified Fe₃O₄ nanoparticles were much more with the prolongation of culture time or the increase of concentration.AAS analysis indicated that the extracellular adhesion of GSSG-modified magnetic particles was depended on culture time and concentration.The uptake amount of GSSG-modiied Fe₃O₄ nanoparticles by SPC-A1 cell was saturated after 24?h when the nanoparticles in low concentration（0.1?mg/ml or 0.4?mg/ml）,the uptake was saturated after 72?h when the nanoparticles in high concentration（0.7?mg/ml or 1.0?mg/ml）.The maximal amount per SPC-A1 cell could reach 160?pg.Conclusion The uptake of GSSG-modified Fe₃O₄ nanoparticles by SPC-A1 cell is energy needed.It depends on the concentration of nanoparticles and saturates at certain time.更多还原