【摘要】 目的研究iNOS、eNOS和HIF-1α在大肠癌和非肿瘤性大肠黏膜中的表达及与血管生成的关系。方法取大肠癌和非肿瘤性大肠黏膜组织各80例制作组织芯片,采用免疫组化技术检测iNOSe、NOS、HIF-1α的表达。结果iNOSe、NOS和HIF-1α在大肠癌和非肿瘤性大肠黏膜中的表达差异极显著(P<0.01);iNOS、HIF-1α阳性组的MVD分别高于各自的阴性组(P<0.05);iNOS的表达与大肠癌分化程度、浸润深度有关(P<0.01);HIF-1α的表达与浸润深度、Dukes分期有关(P<0.05)。结论检测iNOS、HIF-1α在大肠癌中的表达对判断大肠癌的恶性程度有一定价值。应用组织芯片大规模高效检测临床组织样本具有快速、方便、经济、准确的特点。更多还原

【Abstract】 Objective To discuss the expression of inducible nitric oxide synthase(iNOS),endothelial nitric oxide synthase(eNOS),and hypoxia inducible factor-1α(HIF-1α) in human colorectal carcinomas and non-neoplasm colorectal mucosa.Methods The tissue microarrays(TMAs) were made up of 80 cases of colorectal carcinoma and 80 cases of non-neoplasm colorectal mucosa.The expression of NOS and HIF-1α was detected by immunohistochemistry(S-P method).Results The positive rate of iNOS and HIF-1α in colorectal carcinoma was higher than that in non-neoplasm colorectal mucosa;the positive rate of eNOS in colorectal carcinoma was lower than that in normal colorectal mucosa.The expression of iNOS was correlated with differentiation and with invasive depth.But the positive rate of eNOS did not have this feature.The expression of HIF-1α was correlated with infiltrative depth and Duke’s staging.The micro vessel density(MVD) of iNOS positive group was higher than that of negative group and so did HIF-1α. The MVD between different eNOS groups had no difference. Conclusion Over-expression of iNOS and HIF-1α in colorectal carcinoma is correlated with the biological characteristics such as differentiation and invasive depth;It also correlates with MVD.To inhibit the expression of iNOS or HIF-1α might be an approach to inhibit angiogenesis in colorectal carcinoma.It is feasible to detect a lot of samples efficiently by using tissue microarray,because it is fast,convenient,economic and accurate.更多还原