Trauma and pulmonary thromboembolism:an experimenta study on their correlation

【摘要】 <正>Objective:To investigate the correlation between trauma and pulmonary thromboembolism. Methods:Comminuted fractures and extensive soft- tissue contusion at both hind limbs were made by a falling weight from a height in 16 rabbits.Lung perfusion scanning was performed to obtain the radioactivity counts before trauma,at 1 h,48 h and 96 h after trauma.All the data were divided into 4 groups based on the above 4 time points.The rabbits were sacrificed when positive findings on the pulmonary perfnsion scanning appeared.Their lungs were harvested to be paraffin-embedded and stained with hematoxylin-erosin method for histological examination of thromboembolism.The randomized block design ANOVA and the method of least significant difference(LSD)were used for statistical analysis of the radioactivity counts. Results:The histological findings showed that pulmonary embolism developed in 6 of the 16 rabbits (37.5%).Five of the 6 pulmonary embolism rabbits presented neither clinical symptoms nor positive pulmonary embolism manifestations in the lung perfusion scanning.A significant difference was found in lung perfusion radioactivity between the pre-traumatic,post-traumatic 1h groups and post-traumatic 48 h and 96 h groups(P<0. 05). Conclusions:Fractures of the hind limbs accompanied with extensive soft-tissue contusion may cause pulmonary micro-embolism that is not sensitive to lung perfusion scanning and tends to have no clinical symptoms. Pulmonary embolism development may take more than two days after trauma.更多还原

【Abstract】 Objective:To investigate the correlation between trauma and pulmonary thromboembolism. Methods:Comminuted fractures and extensive soft- tissue contusion at both hind limbs were made by a falling weight from a height in 16 rabbits.Lung perfusion scanning was performed to obtain the radioactivity counts before trauma,at 1 h,48 h and 96 h after trauma.All the data were divided into 4 groups based on the above 4 time points.The rabbits were sacrificed when positive findings on the pulmonary perfnsion scanning appeared.Their lungs were harvested to be paraffin-embedded and stained with hematoxylin-erosin method for histological examination of thromboembolism.The randomized block design ANOVA and the method of least significant difference(LSD)were used for statistical analysis of the radioactivity counts. Results:The histological findings showed that pulmonary embolism developed in 6 of the 16 rabbits (37.5%).Five of the 6 pulmonary embolism rabbits presented neither clinical symptoms nor positive pulmonary embolism manifestations in the lung perfusion scanning.A significant difference was found in lung perfusion radioactivity between the pre-traumatic,post-traumatic 1h groups and post-traumatic 48 h and 96 h groups(P<0. 05). Conclusions:Fractures of the hind limbs accompanied with extensive soft-tissue contusion may cause pulmonary micro-embolism that is not sensitive to lung perfusion scanning and tends to have no clinical symptoms. Pulmonary embolism development may take more than two days after trauma.更多还原