【摘要】 目的:研究我国胃癌高发区IL-1B-511基因多态性、幽门螺杆菌(Hp)感染与胃黏膜萎缩的关系。方法:研究对象为胃癌高发区(陕西)健康志愿者500例,人群按照年龄分成20-29岁、30-39岁、40-49岁、50-59岁、60岁以上等5组,每组100例。IL-1B-511基因多态性的分析采用限制性片段长度多态性(PCR-RFLP)方法,以血清胃蛋白酶原Ⅰ(PGⅠ)浓度和PGⅠ/PGⅡ比值作为胃黏膜萎缩的指标,血清PGⅠ、PGⅡ浓度和抗Hp-IgG抗体的检测均采用酶联免疫吸附试验(ELISA)检测。结果:随着年龄的增长,血清PGⅠ的浓度与PGⅠ/PGⅡ的比值呈逐渐下降的趋势;在各年龄组中,IL-1B-511T/T基因型Hp阳性者其血清PGⅠ及PGⅠ/PGⅡ低于相应年龄组Hp阴性者(P<0.05);多因素回归分析提示"IL-1B-511T/T基因型"、"年龄段"和"Hp"对"PGⅠ/PGⅡ比值"有显著影响(P<0.05、P<0.05和P<0.01)。结论:我国胃癌高发区胃黏膜萎缩的发生与IL-1B-511T/T基因型、年龄和Hp感染有密切关系,IL-1B-511T/T基因型增加Hp感染后胃黏膜萎缩发生的危险性。更多还原

【Abstract】 AIM: To study the relationship between IL-1B-511 single nucleotide polymorphism, H. pylori infection, and gastric atrophy in high prevalent (Shanxi) region in China. METHODS: Five hundred healthy volunteers from Shanxi Province of China were recruited in this study, which were divided into five subgroups according to age, namely age 20-29, 30-39, 40-49, 50-59 and >60 years, respectively. Genomic DNA was extracted from peripheral blood. IL-1B-511 single nucleotide polymorphism was analyzed by PCR-RFLP. Serum pepsinogen was used as a biomarker of gastric atrophy. Serum pepsinogen Ⅰ (PGⅠ), pepsinogen Ⅱ (PGⅡ) and anti-H. pylori IgG were determined by an ELISA assay. RESULTS: The mean serum PGⅠ concentration and ratio of PGⅠ /PGⅡ decreased gradually with increasing age, and were lower in subjects with IL-1B-511 T/T genotype and H. pylori infection than those without H.pylori infection in each subgroup, respectively (All P<0.05). Multiple linear regression showed that IL-1B-511 T/T genotype, age and Hp infection were significantly associated with gastric atrophy (P<0.05, P<0.05 and P<0.01, respectively). CONCLUSION: Gastric atrophy is closely correlated with IL-1B-511 T/T genotype, age, H. pylori infection in high prevalence region of gastric cancer in China. The risk of gastric atrophy is significantly increased for the IL-1B-511 T/T genotype with Hp infection.更多还原