【摘要】 目的:探讨肺缺血再灌注损伤Fas/FasL系统的表达及其与肺泡上皮细胞凋亡的关系及川芎嗪的影响。方法:采用在体兔单侧肺左肺门持续性阻断1 h,再灌注1、3、5 h缺血-再灌注损伤的动物模型。TMP干预组于缺血前1h静脉滴注川芎嗪60 mg/kg。用原位末端标记法(TUNEL)检测细胞凋亡的发生情况;用原位杂交的方法检测兔肺组织Fas/FasL mRNA的表达。结果:IR组与TMP组在肺缺血再灌注后1、3、5 h发生明显的肺泡上皮细胞凋亡。TMP组各时相细胞凋亡指数明显低于IR组(P<0.01);肺组织Fas/FasL mRNA的表达与肺泡上皮细胞凋亡呈显著正相关(r1=0.900,r2=0.869,均P<0.01)。结论:Fas/FasL系统在肺缺血再灌注诱导的肺泡细胞凋亡中起着重要作用,川芎嗪因抑制Fas/FasL,而减轻由Fas/FasL系统激活导致的细胞凋亡,从而对缺血再灌注肺具有保护作用。更多还原

【Abstract】 AIM: To study the expression of Fas/FasL mRNA in lung tissue with ischemia-reperfusion lung injury in rabbits and the relationship with the apoptosis,and to observe the effects of Tertram ethylpyrazine on them.METHODS: The pulmonary ischemia-reperfusion models in rabbits with occlusion of left pulmonary hilum for 1 h and then reperfusion 1,3,5 h respectively were used in this experiment.In TMP group,Tertram ethylpyrazine was intravenously dropped at dose of 60 mg/kg at 1 h before ischemia.The TUNEL technique was used to explore apoptotsis of pulmonary cells.In situ hybridization was performed on the rabbit lung tissue to assay the expression of Fas/FasL mRNA.RESULTS: Apoptosis of pulmonary cells was found in both IR group and TMP group.Compared with group IR,the apoptosis index(AI) was decreased obviously in group TMP(P<0.01).There was a significant positive correlation between the expression of Fas/FasL mRNA and the apoptosis of pulmonary cells(r1=0.900,r2=0.869,P<0.01).CONCLUSION: The activation of Fas/Fas-L system may contribute significantly to induce pneumocyte apoptosis in pulmonary ischemic injury.Tertram ethylpyrazine inhibits the activation of Fas/FasL system to decrease apoptosis in pulmonary tissue,which may protect the pulmonary tissues in ischemia injury.更多还原