【摘要】 目的:观察电针对脑梗塞大鼠缺血半影区星形胶质细胞形态学的影响,为探讨针刺治病疗效和机制提供实验依据。方法:线栓法建立大鼠脑梗塞动物模型,用免疫组织化学方法显示胶质纤维酸性蛋白(GFAP)免疫阳性细胞,对比观察电针对模型大鼠脑皮质缺血半影区星形胶质细胞的形态影响。结果:免疫组织化学方法显示电针对脑梗塞缺血半影区星形胶质细胞的数量、细胞面积比与对照组相比差异无显著意义。结论:频率5-10 Hz和强度2 mA的电针穴位刺激没有影响缺血半影区内星形胶质细胞的形态,但是对GFAP的表达有上调的影响。更多还原

【Abstract】 AIM: Effect of electron acupuncture on astrocyte morphology of cerebral ischemic penumbra zone in rats was observed.METHODS: The brain infarct model in male Sprague-Dawley rats was made by thread embolism cerebral middle artery.Immunochemistry method was used to observe the change of astrocyte morphology in cerebral ischemic penumbrazone.RESULTS: There was no statistically difference in astrocyte morphology between cerebral infarct group and electroacupuncture treatment group.CONCLUSION: Acupoint stimulation of 5 and 10 Hz electro-acupuncture with a current intensity of 2 mA may not affect on astrocytic morphology.更多还原