【摘要】 目的:观察阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者胰岛素样生长因子-2(IGF-2)水平的变化,并探讨其与OSAHS及其并发的心血管疾病的关系。方法:选择40例OSAHS患者作为病例组,20例正常人为对照组,病例组再根据血压水平分为正常血压及高血压组,采用酶联免疫吸附试验(ELISA)检测外周血血清中IGF-2水平,用RT-PCR技术检测受试者外周血单个核细胞胰岛素样生长因子-2mRNA(IGF-2mRNA)水平,并对病例组中15例呼吸暂停低通气指数(AHI)>40次/h的患者进行经鼻持续气道正压通气(nCPAP)治疗1月后,重复以上过程。结果:OSAHS患者血清IGF-2水平及外周血单个核细胞IGF-2 mRNA水平明显高于对照组(P<0.05),并与血压升高水平相关。进一步分析与患者AHI呈正相关,与小于90%血氧饱和度时间占总睡眠时间百分比呈正相关,与呼吸暂停时间占总睡眠时间百分比呈正相关,与平均血氧饱和度、最低血氧饱和度呈负相关。经nCPAP治疗后,重度OSAHS患者血清IGF-2水平及外周血单个核细胞IGF-2 mRNA水平明显降低,差异有显著意义。结论:OSAHS患者IGF-2水平明显增高,增高的IGF-2可能参与OSAHS的病理过程,特别是OSAHS引发心血管疾病的相关病理生理过程。更多还原

【Abstract】 AIM:To observe the change of insulin-like growth factor-2(IGF-2)in patients with obstructive sleep apnea-hypopnea syndrome(OSAHS),and to explore the relationship of IGF-2,OSAHS and cardiovascular disease complicated with it.METHODS:The level of serum IGF-2 in 40 OSAHS patients and 20 healthy controls was measured by enzyme-linked immunosorbent assay(ELISA).The expression of IGF-2 mRNA in peripheral blood mononuclear cells was detected by reverse transcription polymerase chain reaction(RT-PCR).RESULTS:The serum level of IGF-2 and the expression of IGF-2 mRNA in peripheral blood mononuclear cells were significantly higher in OSAHS group than those in control group(P<0.05)and IGF-2 related with severity of OSAHS.There was a positive correlation between IGF-2 level and apnea hypopnea index(AHI)as well as average angiosthenia.Also,there was a positive correlation between IGF-2 level and percentage of time spend when oxygen saturation was lower than 90%(SLT90%),and so did between IGF-2 level and the ratio of apnea time over total sleep time.There was a negative correlation between IGF-2 and average saturation of blood oxygen as well as lowest saturation of blood oxygen.After nCPAP,the level of IGF-2 in OSAHS patients reduced significantly.CONCLUSION:The level of IGF-2 in OSAHS increases,and it may play a role in the pathophysiology of OSAHS and the formation of cardiovascular disease complicated with it.更多还原