【摘要】 目的:观察6月龄的快速老化系SAM-P/8、SAM-R/1小鼠与昆明系小鼠的AD相关指标的变化。方法:取健康(20±5)g6月龄SAM-P/8小鼠、SAM-R/1小鼠和昆明系小鼠各14只,雌雄各半,随机分成:SAM-P/8小鼠(AD疾病模型小鼠)、SAM-R/1小鼠对照组和昆明系小鼠对照组,观察上述3组小鼠行为学、神经生化、超微结构、基因表达情况。结果:6月龄SAM-P/8小鼠1-4d学习成绩、第5d记忆成绩低于6月龄SMA-R小鼠和昆明系小鼠(P<0.05),真性胆碱酯酶活性则高于6月龄SMA-R/1小鼠和昆明系小鼠(P<0.05);SAM-P/8小鼠海马神经元超微结构显示明显纤维化,而SAM-R/1小鼠和昆明系小鼠无明显纤维化;SAM-P/8小鼠脑神经细胞的凋亡相关基因表达有明显上调达2倍以上,而SAM-R/1小鼠和昆明系小鼠则未见这些基因有明显上调。SAM-R/1小鼠和昆明系小鼠比较,除基因表达略有差异之外,其它指标无明显差异。结论:6月龄SAM-P/8小鼠在很多方面已经具备了AD自然发病模型典型特征。而用于正常对照的6月龄SAM-R/1与昆明系小鼠的行为学、TchE活性、超微结构则无显著差异;凋亡相关基因表达差异不明显,而部分基因表达则有一定差异。更多还原

【Abstract】 AIM: To investigate the differences of Alzheimer’s disease(AD)-related parameters in the SAM-P/8, the SAM-R/1 and the Kunming mice.METHODS: The changes of ethology, neurobiochemistry (true choline esterase, TchE), ultrastructure and gene expression(gene chips) were determined in mice of three groups: SAM-P/8 mice (n=14), SAM-R/1 mice (n=14) and Kunming mice (n=14), which were 6 months old[weight(20±5)g].RESULTS: The SAM-P/8 mice had the inabilities of learning and memory compared with the SAM-R/1 mice and the Kunming mice (P<0.05). The activity of TchE in the SAM-P/8 mice was higher than that in the SAM-R/1 mice and the Kunming mice (P<0.05). Moreover, neurodegeneration was observed in the hippocampus neuron, and the apoptotic gene expression was up-regulation in mice of SAM-P/8 group. In constrast, neurodegeneration and the up-regulation of the apoptotic gene expression in the SAM-R/1 mice and the Kunming mice were not observed.There were some differences in expression of a few genes between the SAM-R/1 and the Kunming mice except for the apoptotic gene expression.Howerer, There was no difference among abilities of learning and memory, TchE and ultrastructure in SAM-R/1 and Kunming mice.CONCLUSION: The SAM-P/8 mice show many characteristics like Alzheimer’s disease that are significantly different from those in SAM-R/1 mice and Kunming mice. In some circumstauces, Kunming mice can also serve as control in the AD study.更多还原