【摘要】 目的:研究垂体腺苷酸环化酶激活肽(PACAP)对冠状动脉粥样硬化(AS)形成过程中家兔心脏重塑的干预。方法:雄性新西兰家兔60只,随机等分为正常对照组(C组)、AS模型组(A组)及PACAP干预组(P组)。于实验第4、8、12周末每组随机处死动物5-6只,定位取带有冠脉主干及分支的心脏组织制作石蜡切片,分别行HE、van Gieson染色,在光镜下进行定性观察和/或定量分析。结果:(1)C组心脏组织无病变。A组及P组冠状动脉的主干和小分支内均有斑块形成,心肌间胶原成分增多;但P组冠脉小分支内病变及相应部位胶原沉积较同期A组的轻。(2)A组冠状微动脉重塑指数增大,12周末时(2.58±1.54)显著高于同期C组(1.34±0.58)和P组(1.39±0.48)(P<0.05);心肌细胞最大横径缩小,8周末时(13.85μm±2.27μm)已显著小于同期C组(14.68μm±2.40μm)(P<0.05),12周末时显著小于同期C组和P组。(3)P组的上述相应参数与C组无明显差异(P>0.05)。结论:PACAP可抑制家兔冠状AS形成过程中存在的冠状动脉及心肌形态不良改变。更多还原

【Abstract】 AIM: To study the intervention effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on cardiac remodeling during the development of rabbit coronary atherosclerosis. METHODS: 60 male New Zealand rabbits were equally divided into 3 groups randomly: control group (C group), atherosclerosis model group (A group) and PACAP intervention group (P group). At the 4th, 8th and 12th week, 5-6 cases of rabbits in each group were sacrificed, cardiac tissue with coronary arteries were harvested to make paraffin sections. The sections were stained with hematoxylin-eosin and van Gieson separately. The qualitative observation and/or quantitative analysis were made by light microscope. RESULTS: (1)There was no lesion in C group. For A group and P group, there were plaques in large epicardial coronary arteries and small coronary arteries; an impressive accumulation of collagen was also observed in myocardium. In P group, the lesions of small coronary arteries were less serious, and the degrees of perivascular and myocardial fibrosis also appeared to be less.(2)For A group, the wall-to-lumen ratios in small coronary arteries were significantly greater at the 12th week (2.58±1.54) than C group (1.34±0.58) and P group (1.39±0.48) (P<0.05); and the width of cardiomyocyte (13.85 μm±2.27 μm) was already remarkably narrower than C group (14.68 μm±2.40 μm) at the 8th week (P<0.05) and narrower significantly than C group and P group at the 12th week. (3)There were not difference significantly between the above-related parameters of P group and C group (P>0.05). CONCLUSION: Structure changes exist in coronary arteries and myocardium during the development of rabbit coronary atherosclerosis, PACAP can inhibit the cardiac remodeling.更多还原