【摘要】 目的:探讨阿魏酸钠对β淀粉样肽(Aβ25-35)介导的p38信号转导的影响。方法:培养小鼠腹腔巨噬细胞,采用Western印迹分析p38 MAPK蛋白激酶水平。用ELISA法、免疫组化法、Griess反应,分别检测TNF-α生成量、iNOS表达和NO含量。结果:Aβ25-35能诱导p38 MAPK的活化,显著增加腹腔巨噬细胞TNF-α、iNOS和NO水平,阿魏酸钠能显著降低Aβ25-35诱导的腹腔巨噬细胞核蛋白p38 MAPK的含量及细胞的TNF-α、iNOS、NO水平。结论:阿魏酸钠可抑制Aβ25-35介导的p38 MAPK活性,使TNF-α、iNOS、NO水平降低。更多还原

【Abstract】 AIM: To investigate effect of sodium ferulate on Aβ25-35-mediated signaling pathway. METHODS: The isolated peritoneal macrophages from mice were cultured. p38 MAPK protein kinase in nuclear extracts was analyzed by Western blotting. The concentration of TNF-α and NO in supernatant were measured by ELISA and Griess reaction technique. The expression of iNOS protein was detected by immunochemical technique. RESULTS: Aβ25-35 significantly increased the concentrations of TNF-α and NO in supernatant, expression of iNOS in macrophages and p38 MAPK protein kinase in nuclear extracts, which were blocked by sodium ferulate. CONCLUSION: Sodium ferulate inhibits p38 MAPK activation triggered by Aβ25-35.更多还原