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氯化钠胁迫对嫁接茄子生长、抗氧化酶活性和活性氧代谢的影响

Effects of NaCl stress on the growth,antioxidant enzyme activities and reactive oxygen metabolism of grafted eggplant

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【作者】 刘正鲁朱月林胡春梅魏国平杨立飞张古文

【Author】 LIU Zheng-lu,ZHU Yue-lin,HU Chun-mei,WEI Guo-ping,YANG Li-fei,ZHANG Gu-wen(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

【机构】 南京农业大学园艺学院南京农业大学园艺学院 南京210095南京210095

【摘要】 以日本引进的茄子设施栽培专用耐盐品种’Torvum Vigor’为砧木,栽培品种’苏崎茄’为接穗,在营养液栽培条件下,对80mmol.L-1NaCl胁迫下茄子嫁接苗和自根苗的光合特性、叶片抗氧化酶活性、活性氧代谢进行了比较.结果表明:NaCl胁迫下,茄子嫁接苗的干质量、叶绿素含量和光合速率分别比苏崎茄自根苗高67.8%、8.8%和31.1%,均有显著差异;抗氧化酶活性均显著高于苏崎茄自根苗,而O2.-生成速率、H2O2和MDA含量则显著低于苏崎茄自根苗.NaCl胁迫明显降低了叶片叶绿素含量和光合速率,显著增加了O2.-生成速率、H2O2和MDA含量,但嫁接苗受NaCl胁迫的影响小于苏崎茄自根苗,嫁接苗较强的耐盐性与NaCl胁迫下较高的抗氧化酶活性和较低的氧化损伤有关.

【Abstract】 By the method of hydroponics and with the salt-tolerant eggplant cultivar ’Torvum Vigor’(Solanum torvum)from Japan as rootstock and the cultivar ’Suqiqie’(Solanum melongena L.)as scion,this paper studied the differences between grafted and own-root seedlings in their photosynthetic characteristics,antioxidant enzyme activities,and reactive oxygen metabolism under 80 mmol·L-1 NaCl stress.The results showed that under NaCl stress,the dry mass,chlorophyll content and photosynthetic rate of grafted seedlings were 67.8%,8.8% and 31.1% higher than those of own-root seedlings,respectively,and the antioxidant enzyme activities were significantly higher while the O2-· producing rate and the hydrogen peroxide(H2O2)and malondiadehyde(MDA)contents were significantly lower in grafted seedlings leaves than in own-root seedlings.For both grafted and own-root seedlings,NaCl stress reduced the chlorophyll content and photosynthetic rate,but increased the O2-· producing rate and the H2O2 and MDA contents significantly.However,the growth of grafted seedlings was less affected by NaCl stress.The stronger salt tolerance of grafted eggplant seedlings was related to their higher antioxidant enzyme activities and less oxidative damage.

【基金】 江苏省科技厅项目(BC2003306,BE2002304);教育部留学回国基金资助项目([2001]498).
  • 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2007年03期
  • 【分类号】S641.1
  • 【被引频次】113
  • 【下载频次】749
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