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纤溶酶产生菌的筛选及酶活力的测定

Screening of Microbial Fibrinolytic Strain and Measurement of Its Enzyme Activity

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【作者】 张立平李术娜袁洪水王世英朱宝成

【Author】 ZHANG Li-ping1, LI Shu-na2, YUAN Hong-shui2, WANG Shi-ying2, ZHU Bao-cheng1,2 (1. The College of Life Science, Hebei University, Baoding 071002, China;2. The College of Life Science, Agricultural University of Hebei, Baoding 071001, China)

【机构】 河北大学生命科学学院河北农业大学生命科学学院河北大学生命科学学院 河北保定071002河北保定071001河北保定071002

【摘要】 利用纤维蛋白平板法筛选纤溶酶产生菌株并测定其纤溶酶活性。从土壤、豆豉、猪血粉中分别取样,分离到30株具有溶纤活性的菌株,其中来自土壤12株,豆豉10株,猪血粉8株。选择来自土壤,并与已报道的菌株菌落特征不同的34,37菌株号进行研究,测得其酶比活力分别为9.05,624U/mg.Pro。

【Abstract】 Fibrin plate method was used to screen fibrinolytic strain and to examine the fibrinolytic enzyme activity. 30 strains were found to possess fibrinolysis activities, in which 12 strains from soil , 10 from fermented soybean, and 8 from dried pig blood powder. For two strains, namely the serial number 34 and 37, which showed different colony characters as compared to that reported previously, a further research was conducted. Experiments showed that the enzyme activities in the two strains were 9.05U/mg·Pro and 624U/mg·Pro respectively.

【关键词】 微生物纤溶酶菌株筛选
【Key words】 MicrobeFibrinolytic enzymeStrainScreening
  • 【文献出处】 药物生物技术 ,Pharmaceutical Biotechnology , 编辑部邮箱 ,2007年01期
  • 【分类号】R96
  • 【被引频次】6
  • 【下载频次】296
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