【摘要】 目的:建立反相高效液相色谱法测定更舒安颗粒中马钱苷含量。方法:色谱柱采用 Agilent ZORBAX SB C₁₈（4.6 mm×250 mm,5μm）;以乙腈-水（15:85）为流动相,流速1.0 mL·min^-1;柱温25℃;于240 nm 波长下检测。结果:在测定条件下,马钱苷在29.6～296.0μg·mL^-1浓度范围内呈良好的线性（r=0.9999）,最低检出浓度为0.1μg·mL^-1。低、中、高3种浓度的方法回收率分别为100.5%,100.2%,101.3%,RSD（n=3）均小于2%。结论:本方法准确、简便、专属性强、灵敏度高,结果稳定,适用于更舒安颗粒的质量控制。更多还原

【Abstract】 Ohjective:A RP-HPLC method was established for the determination of loganin in Gengshuan gran- ules.Methods:The RP-HPLC method was performed with a Agilent ZORBAX SB C₁₈column（4.6mm 250mm, 5μm）at 25℃,and the mobile phase was acetonitrile-water（15:85,v/v）mixture.The flow rate was 1.0 mL· min^-1.The loganin was detected by UV at 240 nm.Results:The linear range of the loganin was 29.6-296.0 μg mL^-1（r=0.9999）,and the limit of detection was 0.1 μg·mL^-1.The method recoveries were 100.5%,100.2% and 101.3%,and RSD（n=3）for low,medium,high were less than 2%.Conclusion:The method is simple,sensi- tive,selective and repetitive,and is suitable for quality control of Gengshu’ an granules.更多还原