【摘要】 采用三抗体夹心法(TAS-ELISA)研究了烟草漂浮苗中烟草花叶病毒(Tobacco mosaic virus,TMV)通过剪叶操作传播的特点。试验表明,剪一次TMV新鲜病叶的带毒剪刀连续剪10棵健康烟苗,剪叶后第30 d花叶病的平均发病率为12.7%。苗盘试验表明,每剪1盘苗后用漂白粉消毒剪刀1次的情况下,在每盘苗加入6株TMV病苗,剪刀剪叶1次,剪叶后第10d平均发病率为13.0%~18.3%,发病株数增加3.5~4.9倍,无症烟苗移栽后15 d,花叶病发病率可达65%~80%。在第1次剪叶前7 d,每盘苗挑选6株用稀释1.0×10-6倍的TMV病叶汁液摩擦接种,第1次剪叶后13 d(接种后20 d)留下病苗和拔走病苗处理带毒率分别为57.8%和37.8%。第3次剪叶后13 d(接种后47 d)带毒率都达到95.6%。在存在病株的情况下,剪叶传播烟草普通花叶病的效率很高。人工气候箱中,剪叶接种1.0×10-6TMV病叶汁液,接种后11~12 d TAS-ELISA可检测出阳性,接种后20~24 d表现花叶症状。更多还原

【Abstract】 In this paper,the spread character of Tobacco mosaic virus through clipping in tobacco float system was evaluated by TAS-ELISA method.The test results showed that:Among 10 tobacco seedlings which clipped by a clipper carried TMV through clipping a mosaic leaf,12.7% seedlings showed mosaic symptom after 30 d.After added 6 mosaic seedlings in a tray,clipped once, the average incidence of mosaic could reach to 13.0%～18.3%,about 65%～80% symptomless seedlings became mosaic after transplanted 15 d.Seven days before first clipping,inoculated 6 seedlings in a tray with 1.0× 10-6 mosaic leaf juice,the seedling TMV-positive percentage of keep mosaic seedling treatment and move mosaic seedling treatment could reach to 57.8% and 37.8% respectively at 13th day after first clipping,both treatment seedling TMV-positive percentage could reach to 95.6% at 13th day after third clipping.In artificial climate chamber,by inoculated with 1.0× 10-6 mosaic leaf juice through clipping,TAS-ELISA could detect positive seedlings at 11～12 d,seedlings show mosaic symptom at 20～24 d.更多还原