【摘要】 目的:观察重组人内抑素(rh-end)对佐剂性关节炎大鼠滑膜组织p53、fas和bcl-2基因表达的影响。方法:采用SYBR GreenⅠ实时荧光定量PCR方法,定量检测该药对佐剂性关节炎大鼠滑膜组织p53、fas和bcl-2 mRNA表达的影响。结果:与模型组相比,rh-end治疗组大鼠滑膜组织fas和bcl-2 mR- NA表达水平增加,p53 mRNA表达水平降低,差异有统计学意义(P<0.01)。结论:rh-end可经fas介导诱导佐剂性关节炎大鼠滑膜细胞凋亡,且不依赖于p53,不能被bcl-2所抑制。更多还原

【Abstract】 AIM:To observe the effects of recombi- nant human endostatin(rh-end)on the expression of p53,fas and bcl-2 genes in synovial tissue in rats with adjuvant arthritis(AA).METHODS:The effects of rh- end on the expression of p53,fas and bcl-2 mRNA in synovial tissue in AA rats were examined quantitatively by SYBR GreenⅠreal-time fluorescent quantitative PCR (FQ-PCR).RESULTS:The expression offas and bcl-2 mRNA in synovial tissue in AA rats treated with rh-end was significantly increased,as compared with model con- trois(P<0.01).However,p53 mRNA expression de- creased significantly in rh-end group than that in control (P<0.01).CONCLUSION:Recombinant human en- dostatin may induce fas-mediated apoptosis of synovial cells in AA rats,which may be independent on p53 and bcl-2.更多还原