【摘要】 目的研究甲状腺相关性眼病(TAO)眼外肌中免疫细胞的分布,探讨TAO的发病基础。方法标本取自TAO受累眼外肌,对照组取自正常眼外肌组织,免疫组织化学染色法分析TAO受累眼外肌中巨噬细胞、HLA-DR阳性细胞、T淋巴细胞亚群和B淋巴细胞的分布和活性。结果TAO受累眼外肌中包含大量巨噬细胞、HLA-DR阳性细胞和少量T细胞,缺少B细胞,其中巨噬细胞数明显增多,为正常对照组的2倍(P<0.01),但无面积差异(P>0.05)。TAO受累眼外肌中大部分T细胞为CD4+T细胞,而正常对照组主要为CD8+T细胞。结论TAO受累眼外肌中巨噬细胞等免疫细胞增多。更多还原

【Abstract】 ObjectiveThyroid associated ophthalmopathy (TAO) is a kind of immunopathogenic disorder.The immunocompetent cells may be important mediators in the local pathogensis of TAO.The distribution of immunocytes in extraocular muscle (EOM) of eye with TAO was studied to explore the pathogenic mechanism of TAO.MethodsEight EOM specimens were collected from 8 patients with TAO during squint surgeries.The specimens in controls were obtained from healthy corpse eyes.The cell distributions of immunocytes,including macrophages,HLA-DR positive cells,T cells and B cells were analyzed quantitatively using SP immunohistochemical method.ResultsAbundant macrophages,HLA-DR positive cells and T cells were found in extraocular muscle of TAO.Whereas B cells were absent in all specimens.There was a significant increase in macrophages in TAO compared with the control(P<0.01).In extraocular muscle of TAO,macrophages were more two times than control tissues (97±10.15/mm2 in TAO,48±7.17/mm2 in control).CD4+ T cells with brown color in cytoplast were evident in TAO specimens and CD8+ T cells in control tissues.ConclusionMacrophages is probably involved in the pathogenesis of TAO.Early intervention of immunosuppressive therapy of anti-macrophage may influence the sequel caused by extraocular muscle fibrosis in TAO.更多还原