【摘要】 用PCR-SSCP技术研究了波尔山羊和徐淮山羊2个群体共147个个体MyoD基因家族中3个基因座位的单核苷酸多态性(single nucleotide polymorphism,SNP)。结果表明,在徐淮白山羊群体中,myf-5基因座发现有3种基因型AA、AB和BB,波尔山羊均为AA型。在myf-6基因座和myoD5′侧翼区基因座,两个山羊群体均检测到了AA和AB型个体。对山羊myf-5、myf-6基因座,myoD5′侧翼区基因座不同基因型与两品种山羊体尺性状相关分析表明,myf-5基因座对管围和管围指数的效应差异显著(P<0.05)。myf-6基因座对徐淮白山羊体尺性状的效应均不显著(P>0.05),而对波尔山羊的体高和管围指数效应差异显著(P<0.05)。两个山羊品种myoD5′侧翼区不同基因型个体的体尺性状差异均不显著。更多还原

【Abstract】 Single nucleotide polymorphisms (SNPs) of hircine myf-5,myf-6 genes and 5′flanking region of the myoD gene (myoD 5′) were analyzed by PCR-SSCP in Boer goat and Xuhuai white goat. Three genotypes were found at the myf-5 lo-cus of Xuhuai white goat,denoted AA,AB and BB,while all of Boer goats were of AA genotype. Only AA and AB genotypes were found at myf-6 and myoD 5′ loci. The effects of genotypes of myf-5,myf-6 and myoD 5′flanking region loci on body measurement traits were estimated. The results showed that genotypes at the myf-5 locus were significantly associated with hucklebone width and hucklebone width index in Xuhuai white goat (P<0.05). Genotypes at the myf-6 locus were signifi-cantly associated with withers heights and hucklebone width index in Boer goat (P<0.05),but no significant associations were found for any traits in Xuhuai white goat. The myoD 5′ locus was not significantly associated with any traits in both types of goat.更多还原