【摘要】 本研究利用属于7个生理小种的8个云南稻瘟病菌株对以南29和中花9号为受体,分别转入水稻外源基因几丁质-葡聚酶基因和溶菌酶基因的T7代和T10代亲本株系、6个杂交F1代及3个基因聚合后的35个花培株系进行接种分析。结果表明,F1代和3个外源基因聚合后花培的所有株系对所用的8个稻瘟病菌株都表现高抗,抗性明显提高,这是3个外源基因互为补充的结果,说明基因聚合可扩大抗谱,提高稻瘟病抗性。更多还原

【Abstract】 Two transgenic lines,T7 harboring binary chitinase and β-1,3-glucanase gene and T10 possessing a lysozyme gene,their F1 plants of 6 crosses as well as 35 plants from anther culture of F1,were used for identification and evaluation of resistance levels to rice blast with 8 blast isolates from Yunnan which belongs to 7 different races of Magnaporthe grisea.The result indicated that F1 and the anther cultural plants with three foreign resistant genes showed high resistant to 8 isolates.The resistance of all the pyramids were enhanced to almost all the races used in present study,due to the complementation of three foreign genes in each line.This implies that pyramiding method can increase the strength of resistance to rice blast.It suggests that gene pyramiding is an effective approach for improvement of rice varieties with durable resistance to blast.更多还原