【摘要】 目的:体外实验观察生物可降解材料乙交酯-丙交酯共聚物与神经生长因子复合支架的细胞相容性及其缓释作用。方法:实验于2005-05/09在首都医科大学附属北京市神经外科研究所损伤修复实验室完成。①实验方法:乙交酯-丙交酯共聚物采用专利技术(200510011350.9)制备,体外将许旺细胞和神经干细胞与乙交酯-丙交酯共聚物共培养。②实验评估:扫描电镜下观察许旺细胞和神经干细胞在乙交酯-丙交酯共聚物内生长状况;应用组织工程技术将神经生长因子整合入乙交酯-丙交酯共聚物内,0.02g乙交酯-丙交酯共聚物(干质量)中含有1μg神经生长因子,ELISA法检测每天神经生长因子释放的质量浓度。结果:①扫描电镜下乙交酯-丙交酯共聚物横断面可见其为网格状,纵断面可见其直孔道。②许旺细胞和神经干细胞在乙交酯-丙交酯共聚物支架上生长良好,与其紧密贴附。③培养后第4天神经生长因子释放的质量浓度达峰值,第7天趋于平稳,稳定释放的质量浓度为100μg/L。结论:①乙交酯-丙交酯共聚物与许旺细胞和神经干细胞具有良好的生物相容性及细胞亲和性。②按神经生长因子(1μg)与乙交酯-丙交酯共聚物(干质量0.02g)的比例合成的神经生长因子-乙交酯-丙交酯共聚物组织工程材料可缓慢稳定释放神经生长因子,达到其可以发挥生物学活性的质量浓度100μg/L。更多还原

【Abstract】 AIM: To in vitro observe the cell compatibility and release of biodegradable poly(lactide-co-glycolide acid) (PLGA) scaffold integrated with nerve growth factor (NGF). METHODS: The experiment was carried out in the Laboratory of Injury Repairs, Beijing Institute of Neurosurgery Affiliated to Capital Medical University from May to September in 2005.①The production of PLGA was made by technology of patent (200510011350.9). Schwann cells and neural stem cells were cultured within PLGA scaffold in vitro.②The growth of Schwann cells and neural stem cells were observed by scanning electron microscope; NGF was integrated into PLGA by tissue engineering technology, 1 μg NGF for 0.02 g PLGA (dry weight); The release concentration of NGF was tested with ELISA. RESULTS: ①Scanning electron microscope examination: Plenty of meshes on T-sect and straight channels on longitudinal section were observed on PLGA.②Schwann cells and neural stem cells grew well and touched tightly in PLGA scaffold.③The concentrations of NGF reached the peak amplitude on the 4th day, and released steadily on the 7th day with the value was 100 μg/L. CONCLUSION: ①PLGA scaffold has well biological compatibility and cellular affinity with Schwann cells and neural stem cells.②With the ratio of 1 μg NGF in 0.02 g PLGA, NGF can constantly release on the level of 100 μg/L, which displays the biological activity.更多还原