【摘要】 目的:观察穴位肥大细胞与针刺效应之间的关系。方法:SD大鼠48只,随机分为正常对照组、穴位针刺组、旁开针刺组、色甘酸钠组、生理盐水组、色甘酸钠+针刺组、生理盐水+针刺组和色甘酸钠+对侧针刺组。采用大鼠尾部痛阈作为效应指标,在体观察针刺“足三里”提插捻转30 min过程中大鼠的甩尾潜伏期;并通过穴位组织切片染色,离体对照针刺前后穴位处局部肥大细胞脱颗粒率的变化,以及色甘酸钠注射对其的影响。结果:手针大鼠“足三里”穴具有显著镇痛作用,效果明显优于针刺旁开对照点;而在色甘酸钠屏蔽穴位肥大细胞的脱颗粒功能后,这种镇痛作用被明显地削弱。针刺后穴位处局部肥大细胞脱颗粒率显著提高;而注射色甘酸钠可以明显减少该脱颗粒现象。结论:穴位组织中的肥大细胞在针刺镇痛效应的产生过程中起着重要作用,肥大细胞脱颗粒参与了针刺镇痛效应的产生过程。更多还原

【Abstract】 Objective: To investigate the role of mast cells in acupuncture analgesia in rats.Methods: A total of 48 Sprague Dawley(SD) rats were divided into normal control,acupoint [("Zusanli"(ST 36)]-acupuncture(Acu),non-acupoint(3 mm left to ST36),disodium cromoglycate(DSC,0.02 g/mL,for acupoint injection),normal saline(NS,for acupoint injection),DSC+Acu,NS+Acu and DSC+contralateral Acu groups,with 6 cases in each group.The latency of tail flick response to heat irradiation was used as the pain threshold."Zusanli"(ST 36) was punctured with filiform needle and stimulated by lifting and thrusting the needle for 30 min.After sacrifice under anesthesia(1% embutal),tissues of ST36 area were sampled,sliced(4 μm),and stained with Toluidine Blue for skin and Neutral Red for muscles.Results: Compared with normal control group,the ratios of pain threshold increased significantly in all the 7 experimental groups(P<0.05),and those of DSC and NS groups were significantly lower than those of acupoint-Acu,non-acupoint,DSC+Acu,NS+Acu and DSC +contralateral Acu groups(P<0.05).Compared with DSC+Acu group,the ratios of NS+Acu and DSC+contralateral Acu groups were evidently higher(P<0.05,0.01).Compared with control group,the degranulation ratio of acupoint-Acu group was significantly higher(P<0.05,0.001 in muscle and skin separately),and the ratio of DSC+Acu group was markedly lower than that of acupoint-Acu group(P<0.01 in skin).Conclusion: Acupuncture of ST36 has a significant analgesia and enhances the degranulation of mast apparently cells,which is weakened by injection of DSC in the acupoint area,suggesting an important role of mast cells in acupuncture-induced analgesia.更多还原