【摘要】 目的:研究抗重组细粒棘球绦虫抗氧化蛋白TPx(rEgTPx)多克隆抗体对天然EgTPx的结合活性,探讨EgTPx在原头蚴内的分布。方法:采集自然感染细粒棘球蚴的绵羊肝脏,在无菌条件下收集包囊内的原头蚴,经消化处理后制备石蜡切片。利用rEgTPx多克隆抗体,以间接免疫荧光法确定抗氧化蛋白EgTPx在原头蚴内的分布。结果:rEgTPx多克隆抗体能够特异性地结合天然EgTPx抗原表位,EgTPx广泛分布在原头蚴的体表皮层、皮下层和钙颗粒细胞内。结论:确定了EgTPx蛋白在细粒棘球绦虫原头蚴内的分布,为研究EgTPx在原头蚴发育的生物功能奠定基础。更多还原

【Abstract】 AIM:To study the localization of the antioxidant protein thioredoxin peroxidase(TPx) of Echinococcus granulosus(EgTPx) in the protoscoleces(PSC) of parasite.METHODS:The protoscoleces of E.granulosus were aspirated and pooled from sheep liver hydatid cysts.After digested by pepsin,the sedimented protoscoleces were used for paraffin sections.The localization of the antioxidant protein TPx of EgTPx was determined by using(polyclonal antibody against rEgTPx) and indirect immunofluorescence staining.RESULTS:Indirect immunofluorescence staining analysis indicated that the rEgTPx was mainly distributed in tegument,subtegument and calcareous corpuscle cells of the protoscolex.CONCLUSION:The wide distribution and large sites of EgTPx in the parasite hare been clearly determined,which will help further investigation into the biological functions and application of TPx protein.更多还原