【摘要】 以大麦栽培品种的茎尖、成熟胚为外植体,研究了不同甘露糖浓度对这些外植体愈伤组织诱导和生长的影响。结果表明:甘露糖浓度为20 g/L时,茎尖的叶片伸长和生根受到明显抑制;甘露糖浓度为10 g/L或15 g/L时,成熟胚的愈伤组织诱导率降低50%,甘露糖浓度为20 g/L或25 g/L时,成熟胚愈伤诱导和生长完全受到抑制,因此在以大麦茎尖和成熟胚为外植体的磷酸甘露糖异构酶(PM I)/甘露糖筛选中,可分别以20 g/L、25 g/L甘露糖作筛选压。另外,在培养早期阶段筛选比较适宜。更多还原

【Abstract】 Explants derived from shoot apices and mature embryos of elite barley cultivars were assayed for their callus induction and growth on culture media containing various concentrations of mannose as carbon sources.The results showed that a clear inhibiting effect on the leaf elongation and rooting of shoot apices in the concentration of 20 g/L mannose.For mature embryos,callus induction and growth rate were inhibited in the presence of 20 g/L or 25 g/L mannose,whereas 10 g/L or 15g/L treatment could effectively result in a reduction of callus formation and growth up to 50%.These results indicated that 20 g/L and 25 g/L mannose were required for inhibition of the explants derived from shoot apices and mature embryos,respectively,when mannose was used for positive selections of transgenic barley plants.In addition,it is more suitable to select during the initial induction and culture of barley explants.更多还原