【摘要】 目的:建立高效液相色谱法测定葛根异黄酮胶囊葛根素含量的方法。方法:采用HPLC外标法,测定以30%乙醇超声溶解提取葛根异黄酮胶囊的葛根素含量,固定相为Kromasil C18,流动相为甲醇-水(25∶75),UV检测波长为250 nm。结果:葛根素溶液在9.66~96.60μg/ml浓度范围内浓度与峰面积成良好线性关系,r=0.9999,平均加样回收率为99.51%,RSD=1.55%(n=6)。结论:方法简便具有良好的准确性和精密性,结果可靠。更多还原

【Abstract】 OBJECTIVE To establish a HPLC method for determination of puerarin in puerariae isoflavones capsule.METHODS 30% alcohol extracted and dissolved puerariae isoflavones capsule with ultrasonic,then puerarin was separated by Kromasil C18 reversed-phase analysis column.The mobile phase was methanol-water=25∶75(v/v);the wavelength of detection was UV 250 nm.RESULTS There was a good linearity from 9.66 μg/ml to 96.60 μg/ml(r=0.999 9) in concentration and peak area of puerarin.The average recovery of puerariae isoflavones capsule was 99.51%,and the relative standard deviation was 1.55%(n=6).CONCLUSION The method is simple,veracity and precision;and the results is reliable.更多还原