【摘要】 目的:探讨实验性精索静脉曲张导致大鼠附睾功能降低的病理生理机制。方法:雄性Wister大鼠建立左侧精索静脉曲张模型,为实验组(n=11)并以假手术为对照组(n=9),免疫组化法检测附睾上皮p53的表达,DTNB法检测附睾中肉毒碱的含量,以5-甲基苯二酚法检测附睾中唾液酸的含量。结果:大鼠精索静脉曲张模型建立后的d28,实验组左侧附睾上皮p53表达显著高于对照组(P<0.05);实验组附睾中唾液酸、肉毒碱含量显著低于对照组(P<0.05)。结论:p53在精索静脉曲张后附睾功能降低的过程中起重要作用。更多还原

【Abstract】 Objective: To investigate the effect of p53 in the pathophysiology of varicocele. Methods: Experimental left varicocele models of 20 adult male Wister rats were obtained by partial ligation of the left renal vein, including control group (n=9) and experimental group (n=11). The epididymis were collected 28 d after the surgery for detecting p53 expression with immunohistochemical method, the contents of sialic acid by spectrophotometry, and the carnitine content with DTNB. Results: The p53 expression of epididymis epithelium was significantly higher (P<0.025), and the content of sialic acid and carnitine in the epididymis were significantly lower (P<0.05) in experimental group than in control group 28 d after the establishment of the left varicocele models. Conclusion: p53 plays an important role in the reduction of epididymis function in the experimental varicocele rats.更多还原