【摘要】 分离提取海洋无脊椎动物贻贝(Crenomytilus grayanus)凝集素,考察其抗HIV活性。采用半乳糖-Sepharose 6B亲和层析和Sephacryl S-200层析分离提取贻贝凝集素(Crenomytilus grayanus lectin,CGL),以光镜检查合胞体抑制试验,以ELSA测定HIVp24抗原表达水平。从海洋无脊椎动物贻贝中分离出的凝集素(CGL),为N-乙酰半乳糖胺/半乳糖(GalNAc/Gal)特异性的凝集素。CGL在27.88mg.L-1浓度时,对HIV诱导细胞病变的抑制达50%;在45.70mg.L-1时,对HIV-1复制的抑制达50%;同时在35.12mg.L-1浓度时,对HIV感染细胞融合的阻断达50%。更多还原

【Abstract】 CGL,a Crenomytilus grayanus lectin was purified from sea mussel C.grayanus,and the anti-HIV activity was assessed.CGL was purified with affinity chromatograph on galactose-Sepharose 6B and following gel filtration on Sephacryl S-200.The inhibition of syncytial cell formation induce by human immunodeficiency virus was determined under microscope,and the reduction of HIV p24 antigen expression level was measured with ELISA.The purified CGL showed GalNAc/Gal specificity.CGL inhibited cytopathic effect induced by HIV-1 and the production of viral p24 antigen.The EC50 values were 27.88 and 47.5 mg·L-1,respectively.CGL could block the cell-to-cell fusion process of HIV infected and uninfected cells with EC50 values of 35.12 mg·L-1.CGL is a GalNAc/Gal specific lectin with anti-HIV activity.更多还原