【摘要】 目的将诊断结核病的蛋白芯片法与金标法及抗酸染色法进行比较,以评价蛋白芯片的临床应用价值。方法用蛋白芯片法、金标法及抗酸染色法检测75例确诊结核病人、30例肺部其它疾病患者和健康体检者的血清标本,并进行相关统计分析。结果蛋白芯片法、金标法及抗酸染色法检出结核分枝杆菌的灵敏度为64%,72%及22.7%,前两者比较无显著性差异(P>0.05),但均高于抗酸染色法(P<0.05)。三者的特异性分别为90%,76.7%及96.7%。结论蛋白芯片法检测结核分枝杆菌,与金标法及抗酸染色法比较,具有简便、快速、敏感性较高和特异性强等优点,是临床辅助诊断结核病的有效方法。更多还原

【Abstract】 Objective To compare with TB protein-chip(PC),dot-immuogold filtration assay(DIGFA) and acid-fast bacilli smear(AFS) for rapid diagnosis of Tuberculosis(TB),and to evaluate the clinical application value of TB protein-chip.Methods 75 serum samples of TB patients,30 non-TB lung diseases patients and healthy control people were detected by TB PC,DIGFA and AFS simultaneously,and the χ2-test was performed.Results The sensitivity of the TB PC,DIGFA and AFS were 64%,72% and 22.7%,respectively.There were no significant difference between the TB PC and DIGFA(P>0.05),which was higher than that of AFS(P<0.05),while the specificity of the three methods were 90%,76.7% and 96.7%,respectively.Conclusion Compared with DIGFA and AFS,the TB protein-chip is simple,rapid,sensitive and specific for detection multiple TB antibodies,which would be taken as a helpful method for early and rapid diagnosis of tuberculosis.更多还原