【摘要】 DNA shuffling技术是一项全新的体外人工进化模式,它通过基因在分子水平上的重组,再定向筛选具有预期性状的突变体,获得同时具有多个亲本基因的特征的突变基因。该文介绍DNA shuffling技术的基本原理,并列举了由该技术发展而来的新技术及其在基因工程疫苗领域的应用,展望了DNAshuffling技术的发展方向。更多还原

【Abstract】 DNA shuffling is a new technology for artificial evolution, gene recombination on molecular level and directed selection are deployed to obtain mutant with expected qualities. Recent development of DNA shuffling and its application on genetically engineering vaccines was reviewed in this article.更多还原