【摘要】 目的:观察不同浓度异丙嗪对大鼠急性前脑缺血再灌注线粒体通透性转换(MPT)的影响,并从分子水平探讨其神经保护作用的机制。方法:用夹闭双侧颈总动脉再开放法制作全脑缺血模型。大鼠随机分为缺血再灌注+异丙嗪组(治疗组),假手术+异丙嗪组(假手术组),缺血再灌注+NS(模型组);术前1 h静注不同浓度异丙嗪(5,15,45 mg/kg),术后6 h 3组均分离前脑线粒体,用分光光度法检测MPT值。结果:模型组分别与治疗组(异丙嗪浓度为5 mg/kg时)和假手术组(异丙嗪浓度为5,15 mg/kg时)比较,模型组MPT值减小更为明显(P<0.01);组内比较,异丙嗪浓度为5,15 mg/kg时比浓度为45 mg/kg时抑制MPT值减小的作用更为显著(P<0.05)。结论:异丙嗪能抑制缺血再灌注所致的MPT值的改变,因而可以通过阻止神经细胞线粒体的损伤发挥脑保护作用。更多还原

【Abstract】 Objective: To observe the effects of promethazine at different concentrations on mitochondrial permeability transition(MPT)of acute ischemia-reperfusion in rat fore-brain,and to explore the neuron-protective mechanism in mole-cular level.Methods: Acute ischemia-reperfusion models in rat brain were made by reopening both sides common carotid artery after clipping.Rats were assigned into ischemia-reperfusion with promethazine group,sham operation with prome-thazine group and ischemia-reperfusion with normal saline group.Promethazine at different concentrations(5,15,45 mg/kg)were injected one hour before operation,the mitochondria were extracted from rat fore-head 6 hours after operation.The MPT value in all the groups was determined by spectrophotometer.Results: Compared with the groups of ischemia-reperfusion with promethazine(5 mg/kg)and sham operation with promethazine(5,15 mg/kg),the MPT value decreased significantly in the ischemiareperfusion with normal saline group(P<0.01).In the same group,the effect of promethazine(5,15 mg/kg)was more obvious,compared with 45 mg/kg(P<0.05).Conclusion: Promethazine at various concentrations can inhibit the changes of MPT value induced by ischemia-reperfusion in rat fore-brain,therefore the neuron-protective effect may be produced by preventing neuron mitochondrial impairment.更多还原