【摘要】 目的观察中药葛根水提液对大鼠慢性酒精性肝损伤的防治作用。方法按Lieber-Decarli配方制备酒精液体饲料,以定量喂饲大鼠的方法,复制酒精性肝损伤模型。32只雄性SD大鼠随机分为正常组、无酒精液体饲料组(对照组)、酒精液体饲料组(模型组)及酒精液体饲料+葛根组(葛根组)。在造模第4周开始灌胃葛根水提液或蒸馏水。8周末取材观察:①肝组织病理变化;②肝组织三酰甘油(TG)含量;③血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和肝组织γ-谷氨酰转肽酶(γ-GT)活性;④肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、一氧化氮合酶(NOS)活性及血清铁(Fe2+)含量。结果模型组大鼠肝组织脂肪变性和炎症浸润明显,血清ALT、AST活性和组织γ-GT活性、TG含量较对照组均显著增加;且肝组织MDA含量、NOS活性及血清Fe2+含量均显著升高,而肝组织SOD显著降低。葛根组的肝组织脂肪变性和炎症浸润均较模型组显著减轻,肝组织γ-GT活性和TG含量显著低于模型组;同时肝组织MDA含量、NOS活性显著低于模型组,而SOD活性显著高于模型组。结论葛根水提液有显著的抗慢性酒精性肝损伤的作用。更多还原

【Abstract】 Objective To study the preventive effects of Pueraria on the alcoholic liver injury in rats. Methods Alcoholic liver injury model in rats was duplicated by feeding Lieber-Decarli feed. 32 SD rats were randomly divided into normal group, control group, model group and Pueraria group. Administration began from 4 weeks modeling and samples were taken at the end of 8th week. The followings were detected: the liver pathology (HE staining and Red oil staining); the content of liver triglyceride (TG); the activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver γ-glutamyltransferase (γ-GT); the activity of liver superoxide dismutase (SOD), nitric oxide synthase (NOS), the content of liver maleic dialdehyde (MDA) and serum Fe2+. Results In the experiment, there were serious hepatic steatosis and inflammation in model group. Activity of serum ATL, AST and liver γ-GT and content of liver TG of the model group were significantly increased compared with the normal group and the control group. There were significant increases in MDA, NOS and Fe2+and significant decrease in SOD. Pueraria can obviously alleviate hepatic steatosis and inflammation in model group. Pueraria can significantly decrease serum ALT, AST, NOS activity, liver γ-GT activity and liver TG, MDA content. And it can also increase liver SOD activity. Conclusion Pueraria has good experimentally preventive effect on the alcoholic liver injury.更多还原