【摘要】 目的研究遗传背景是否为PM2.5致肺损伤的因素之一。方法采用气管滴注的染毒方法,两种品系的小鼠(C57BL/6小鼠和C3H/He小鼠)均予PM2.5染毒,连续染毒2d,最后一次染毒24h后,处死小鼠,取肺组织,并且立即置于液氮中保存。然后运用Affymetrix Mouse430 2.0表达谱基因芯片,分析在两种品系小鼠肺部基因表达的差异情况。结果基因芯片结果表明Chi3l3、Chi3l4、Cxcl2、C4、Hc、Cp和Sirpb1基因在B6小鼠肺部的表达明显高于C3小鼠,而Igh-6、Cap1、Mtap2、Nme7和5830443L24Rik基因的表达明显低于C3小鼠。结论由于两种品系小鼠遗传背景上的差别,导致PM2.5染毒后,在两种品系小鼠肺部基因表达存在明显差异,因此遗传因素可能是PM2.5致肺损伤的易感因素之一。更多还原

【Abstract】 Purpose To testify whether the genetic background is one of the factors on lung injury to PM_2.5. Methods By intratracheally instillation,two strains of mice（C57BL/6 and C3H/He mice） were exposed to PM_2.5 once time per day,for continuously two days.Twenty-four hours after the last time exposure,the mice were killed and the lungs were taken out immediately,and put into the liquid nitrogen.By using Affymetrix 430 2.0 GeneChip,the expression of up-regulated genes and down-regulated genes were examined. Results Expression of the genes of Chi3l3,Chi3l4,Cxcl2,C4,Hc,Cp and Sirpb1 in B6 exposed to PM_2.5 was significantly higher than that in C3 mice exposed to PM_2.5,but expression of the genes of Igh-6,Cap1,Mtap2,Nme7 and 5830443L24Rik in B6 was lower than in C3. Conclusions After exposed to PM_2.5,because of the different genetic background between B6 and C3,the expression of genes between B6 and C3 was significantly different.And heredity might be one of the susceptible factors which could lead to different responses in lung after exposed to PM_2.5.更多还原