【摘要】 目的探讨鼠型沙眼衣原体(Chlamydia muridarum,C.muridarum)在树突状细胞(Dendritic Cell,DC)内的存活情况以及对DC功能的影响,为衣原体致病机制的研究提供实验依据。方法诱导培养BALB/c小鼠骨髓来源的DC。用C.muridarum感染DC,分别在247、2 h固定细胞进行衣原体包涵体染色;采用ELISA方法检测感染DC上清中的细胞因子;感染DC与T细胞共孵育48 h,CCK-8检测T细胞增殖。结果C.muri-darum能够感染骨髓来源DC,衣原体在DC中生长缓慢,形成非典型的包涵体;感染后的DC分泌较高水平的IL-12和低水平的IL-10;并且能够刺激T细胞增殖,说明衣原体感染的DC仍然具有抗原递呈能力。结论成功建立C.muridarum感染DC的体外细胞模型,且沙眼衣原体感染对DC抗原递呈功能没有造成明显影响。更多还原

【Abstract】 Objective To explore the survival of Chlamydia muridarum in dendritic cells(DCs) and its effect on the function of DCs so as to provide a basis for immunopathology caused by Chlamydia trachomatis infection.Methods Bone marrow-derived DCs,which were generated from female BALB/c mice were infected by Chlamydia muridarum,and 24 hours and 72 hours later,fixed cells were separately stained with the anti-Chlamydia LPS.Cytokines in the supernatants of the DCs were determined by ELISA;DC and T cells were co-cultured 48 hours,and T cell proliferation was assayed using CCK-8 kit.Results Chlamydia muridarum survived in DCs and atypical inclusions developed.After infection with Chlamydia muridarum,DCs secreted a high level of IL-12 and a low level of IL-10;DCs also stimulated T cell proliferation,which illustrated that infected DCs had the ability to present antigens.Conclusions The cell model of Chlamydia muridarum infecting DCs has been successfully established.After being infected by Chlamydia muridarum,DCs can still present antigens.更多还原