【摘要】 目的:研究王不留行Vaccaria segetalis(Neck)Garcke(VSG)水煎剂对主动脉环的舒张作用及机制。方法:采用家兔离体主动脉平滑肌标本,以去甲肾上腺素(NA)预收缩后,给予不同剂量的VSG观察其张力变化,并探讨去除血管内皮、给予NO合酶抑制剂左旋硝基精氨酸(L-NNA)、甲烯蓝(MB)、环氧酶抑制剂吲哚美辛和β肾上腺素能受体阻断剂普萘洛尔对血管张力变化的影响。结果:VSG对血管环静息张力无明显影响,但不同剂量的VSG可使10-6mol/L NA预收缩的血管产生明显舒张(r=0.76,P<0.001)。去除血管内皮、10-4mol/L L-NNA、10-5mol/LMB或吲哚美辛可减弱VSG的舒张作用,但普萘洛尔无影响。另外,2mg/ml VSG温育无内皮的血管环后,对NA和KCl的量效曲线无明显影响。结论:VSG舒张主动脉的作用是内皮依赖性的,且与其释放的NO有关;也可能是通过PGI2/cAMP介导,降低胞浆内钙离子引起血管平滑肌舒张。更多还原

【Abstract】 Objective: To study the vasodilation effects of the water decoction of Vaccaria Segetalis(Neck) Garcke(VSG) on rabbit aortic rings and to discuss the possible involved mechanism.Methods: We investigated the response to different concentration of VSG in isolated rabbit’s aortic rings precontracted with noradrenaline(NA).Then we assessed the effects on denuded endothelium,coincubated with nitric oxide synthase inhibitor Nw-nitro-L-arginine(L-NNA),methylene blue(MB),prostaglandin synthase inhibitor and blockage of the adrenergic β-receptor,propranolol on VSG-induced relaxation.Results: VSG had no effects on the resting tension of rabbit aortic rings.However,it induced a significantly concentration-dependent relaxation on NA(10-6mol/L) precontracted rings(r=0.76,P<0.001),which could be reduced by denuding endothelium or by 10-4mol/L L-NNA,10-5mol/L MB and prostaglandin synthase inhibitor.But propranolol,the blockage of the adrenergic β-receptor,showed no effects.Furthermore,the concentration-response curves of NA and KCI had not been shifted with VSG(2mg/ml) on the rabbit aortic rings denuded endothelium.Conclusion: There can be seen an endothelium-dependent vasorelaxation on the isolated rabbit’s aortic rings with VSG,and nitric oxide(NO) might be involved in.The signal transduction pathway PG12-cAMP-iCa+ also might be involved in.更多还原