【摘要】 目的:探讨针刺抗脑缺血再灌注炎性损伤的机制。方法:采用线栓法制作大鼠大脑中动脉缺血模型(MCAO),采用免疫组织化学技术研究P-选择素和E-选择表达的动态变化。结果:再灌注3h各实验组P-选择素和E-选择素均开始表达,于再灌注24h均达到高峰(组内比较P<0.01),持续至再灌注48h,针刺可显著降低P-选择素和E-选择素的表达(与模型组和非穴组比较,P<0.01)。结论:P-选择素和E-选择素的表达上调与再灌注后的炎症反应有关,早期针刺治疗可能通过下调脑缺血区粘附分子P-选择素和E-选择素的表达而防治脑缺血再灌注炎性损伤。更多还原

【Abstract】 Objective:To study mechanisms of early treatment on cerebral ischemia-reperfusion(I/R)by acupuncture in resisting inflammation injury in rats.Methods:Cerebral I/R Model was established occlusion of the middle cerebral artery for 1hr and then removal of the occlusion for 3hr,12hr,24hr and 48hr respectively.The DNA expression of P-selectin and E-selectin was displayed by using Imunohistochemical ABC in ischemic cerebral region in rats.Result:The DNA expression of P-selectin and E-selectin was detected at capillary walls in postichemia cerebral cortex at 1hr ischemia-3hr reperfusion and peaked at 1hr ischemia-24hr reperfusion and increased continually during 48hr reperfusion.The DNA expression of both P-selectin and E-selectin in ischemic cerebral cortex were down-regulated considerably by acupuncture treatment(P<0.01).Conclusion:The protective effect of acupuncture on cerebral neurons under acute infarction is possiblely through its downregulative action on the expression of P-selectin and E-selectin,which caused neutrophil infiltration induced by adhesive molecules between endothelium cell and neutrophil.更多还原