【摘要】 目的探讨天麻在大鼠肾缺血再灌注损伤中的保护作用及其机制。方法建立大鼠急性肾缺血再灌注损伤模型,测定天麻对肾缺血再灌注后血清BUN浓度、肾组织SOD活力、MDA及NO含量的变化及肾脏的病理组织学改变。结果与对照组相比,使用天麻后,在灌注24h后血清BUN浓度明显下降(P值<0.01),再灌注1、6、12和24h后肾组织SOD活力明显升高(P值分别<0.01,0.05,0.01),MDA含量明显降低(P值分别<0.05,0.01,0.05);未应用天麻保护的对照组肾组织出现明显的损伤性改变。结论天麻在大鼠肾缺血再灌注损伤模型中有保护作用,其机制可能与天麻的抗自由基损伤和减轻脂质过氧化的作用有关。更多还原

【Abstract】 Objective To study the anti-oxidant effects of Rhizoma Gastrodiae on rat models with renal ischemia reperfusion injuries. Methods Rhizoma Gastrodiae was given intraperitoneally to rat for every 6 hours beginning from 2 days before surgery until 1,6,12 and ,24 hours after reperfusion.At 1, 6, 12 and 24 hours after reperfusion, SOD and MDA in renal tissues were determined, and at 6 and 24 hours after reperfusion renal NO level and serum concentration of BUN were analyzed. Results 6, 12 and 24 hours after reperfusion, the kidney SOD activities were significantly increased (P<0.01, P<0.05 and P<0.01, respectively),while the concentration of renal MDA significantly decreased (P<0.05,P<0.01 and P<0.05, respectively). Serum BUN level was significantly declined at 24 hours after reperfusion (P<0.01). Conclusion Rhizoma Gastrodiae may have preventive role in renal ischemia reperfusion injury, possibly through anti-oxidative actions against free radicals and lipid peroxides.更多还原