【摘要】 目的探讨出入境人群HCV的基因分型,并分析HCV不同基因型与病毒载量、ALT活性水平的相互关系。方法对72542名出入境人员进行HCV抗体检测,对抗-HCV阳性标本进行HCV基因分型,以荧光定量PCR测定HCV RNA载量,同时检测ALT活性。结果共发现197份标本呈抗-HCV阳性,阳性率为0．27％,其中出境人员阳性率为0．24％,入境人员阳性率为0．31％。抗-HCV阳性标本经PCR检测基因分型后发现110份标本含有HCV RNA,共发现5种基因型,其中1b占40．0％、6a占30．0％、2a占14．5％、1a占8．2％、2b占7．3％。中国大陆人群与中国香港及其他国家人群的各基因型分布无明显差异。各基因型群间与HCV RNA载量差异具有统计学意义(P＜0．05),2a型人群HCV RNA载量明显高于其他各型人群(P＜0．05)。不同基因型人群间ALT水平差异无统计学意义。HCV RNA拷贝量与ALT活性无相关性。结论HCV-1b为优势基因型。对HCV基因分型以及PCR病毒载量和ALT活性检测,实行HCV实时监测具有重要的意义。更多还原

【Abstract】 Objective To investigate correlation of HCV Genotype with viral load and ALT from anti-HCV positive international travellers.Methods Anti-HCV test was performed in 72 542 international travelers.The anti-HCV positive samples were test- ed for their genotypes,HCV RNA loads and ALT activities.Results A total of 197 anti-HCV positive samples were detected.0. 27% of all samples were positive with the incidence rates in exit people and entry people were 0.24% of and 0.31%,respeetively. HCV RNA was observed in 110 anti-HCV positive samples using PCR amplification.There five genotypes were identified including the most common genotype 1b(40.0%),6a(30.0%),2a(14.5% ),1a(8.2%)and 2b(7.3%).The HCV RNA load among the five genotypes was statistically significant(P＜0.05).The viral load of 2a group was more than other groups.There no signifi- cant differences were observed in the ALT levels among different genotype groups.There was no correlation between HCV RNA load and ALT activity.Conclusion HCV-1b is the predominant in international travelers.HCV genotyping and determination of HCV RNA load and ALT activity are important mode for monitoring infectious diseases in international travelers.更多还原