【摘要】 采用4,4’-二异氰酸酯二苯基甲烷作键合剂,制备了键合型纤维素苯甲酸酯手性固定相(Bonded-CTB),并在商品化的ChiralcelOD-H、ChiralcelOJ-H手性柱和自制Bonded-CTB手性柱上研究了萘丙胺对映异构体的拆分,考察了流动相正己烷中乙醇、异丙醇和四氢呋喃含量对拆分的影响,探讨了其手性拆分机理。结果表明,萘丙胺在3种手性柱上均得到了基线分离,在自制手性柱上采用四氢呋喃作为添加剂时仍保持良好的稳定性,与涂覆型商品柱相比,可以选择的流动相范围更广,可用于萘丙胺对映体的定量分析。更多还原

【Abstract】 Using 4,4’-methylenediphenyl diisocyanate as bonding spacer,cellulose benzoate was regioselectively bonded to macroporous silica gel at 6-position of the primary hydroxyl group,and enantioseparation of naproanilide was studied on Chiralcel OD-H,Chiralcel OJ-H and self-prepared bonded cellulose benzoate chiral columns(Bonded-CTB),the influences of ethanol,isopropanol and tetrahydrofuran in mobile phase hexane were examed,and their chiral recognition mechanism was studied. The results show that naproanilide can get baseline separation on this three chiral columns,and the self-prepared bonded cellulose benzoate chiral column can use with tetrahydrofuran(THF) as mobile phase modifier,and suitable for quantitative analysis of naproanilide enantiomers.更多还原