【摘要】 从发病信鸽的体内分离到1株新城疫病毒(Newcastle disease virus,NDV)PB9601,经病毒蚀斑纯化后动物回归试验可产生典型的NDV病变,其鸡胚平均死亡时间(MDT)、一日龄雏鸡脑内接种的致病指数(ICPI)、六周龄鸡静脉接种的致病指数(IVPI)分别为50.5h、1.65和2.36,表明NDV为强毒。对其融合蛋白(F)和血凝素神经氨酸酶(HN)基因分别进行克隆测序,发现F蛋白多肽裂解位点的氨基酸基序为112 KRQKRF 117,符合鸽源NDV强毒序列。F基因分型及同源性比较显示:PB9601与YN-03、TW-98等鸽源毒株属于Ⅵ型,其F基因与疫苗株基因Ⅱ型LaSota的氨基酸同源性为88.6%,与疫苗株基因Ⅰ型V4的氨基酸同源性为91.0%,与传统强毒基因Ⅸ型F48E9的氨基酸同源性为90.6%;与国内鸽分离株GX-05、YN-03和TW-98的氨基酸同源性分别为92.1%、95.5%和98.6%;与国外鸽分离株Capital3-97、Tigre6-99、1166-00、IT-227-82和2736-00的同源性分别为93.3%、91.9%、94.6%、95.1%和93.6%。HN基因氨基酸同源比较显示:PB9601与LaSota、F48E9和V4的氨基酸同源性分别为87.2%、89.2%和87.6%;与国外鸽分离株1166-00、IT-227-82和2736-00的同源性为93.7%、95.5%和92.3%。PB9601与国内外鸽分离株、LaSota、F48E9和V4相比,具有明显的地域性和种属性。更多还原

【Abstract】 One Newcastle disease virus(NDV) isolate named PB9601 and collected from homing pigeon was characterized molecularly and biologically. And the isolate was determined as a virulent strain by the mean death time(MDT) of 50.5 h, the intracerebral pathogenicity index(ICPI) of 1.65, and the intravenous pathogenicity index (IVPI) of 2.36. The fusion(F) and hemagglutinin-neuraminidase(HN) genes of the isolate PB9601 were cloned and sequenced. The deduced amino acid sequence of F gene showed that the virus shared a velogenic motif 112 KRQKRF 117 at the cleavage site. Phylogenetic analysis showed that the viruses derived from pigeon such as PB9601, YN-03 and TW-98 belonged to genotypeⅥ. For the F gene of the isolate PB9601, the homologies of the deduced amino acid sequences were 88.6%, 91.0% and 90.6%, respectively, compared with strains LaSota, V4 and F48E9; 92.1%, 95.5% and 98.6% individually for isolates GX-05, YN-03 and TW-98 from host pigeon in China, and 93.3%, 91.9%, 94.6%, 95.1% and 93.6% respectively for pigeon-origin isolates Capital3-97, Tigre6-99, 1166-00, IT-227-82 and 2736-00 overseas. By comparison of the deduced amino acid sequences of HN gene, the isolate PB9601 with the exotic pigeon-origin strains 1166-00, IT-227-82 and 2736-00 shared higher homologies of 93.7%, 95.5% and 92.3%, and lower homologies of 87.2%, 89.2% and 87.6% with LaSota, F48E9 and V4. The results indicated that PB9601 had some obviously regional and genus traits among NDV strains.更多还原