【摘要】 选720日龄美国王鸽120对,随机分成4组。对照组饲喂基础日粮,实验1、2和3组分别添加0.25、0.75和1.25mg/kg olanzapine。通过构建优化RT-PCR反应体系,以β-actin为内标,研究了olanzapine对美国王鸽催乳素受体(PRLR)mRNA表达量的影响,结果显示olanzapine能降低PRLR mRNA的表达量,与对照组相比,添加olanzapine 1.25mg/kg组PRLR mRNA表达量降低了14.3%(P<0.05)。结果表明,日粮添加olanzapine能显著抑制PRLR基因的表达。揭示了olanzapine作为DA(多巴胺)和5-HT(5-羟色胺)受体阻断剂能有效减少就巢行为的发生,缩短就巢时间,提高产蛋量。更多还原

【Abstract】 A hundred and twenty couples of laying pigeons of 720 days of age were randomly divided into four groups.The doves were kept in individual cages and provided with purified diet randomly.The difference of control and treatment groups diet was the amount of olanzapine(the diet of control group contained 0 mg olanzapine/kg and the diet of treatment groups contained 0.25,0.75 and 1.25 mg/kg olanzapine,respectively).The effects of olanzapine on the expression of prolactin receptor(PRNA)gene were investigated by RT-PCR.1.25 mg/kg olanzapine decreased the relative mRNA expression of PRLR/β-actin by 14.3%(P <0.05).These results implicated that olanzapine could significantly increase egg production by inhibiting the expression of PRLR gene from pituitary gland.These results also indicated that hypothalamic DA(dopamine)and 5-HT(5-hydroxytryptamine)activities were the vital causes of development and maintenance of broodiness and pituitary prolactin(PRL)secretion.更多还原