【摘要】 目的:观察动物模型肝包虫囊壁情况,研究骨桥蛋白(Osteopontin,OPN)在小鼠肝细粒棘球蚴外囊壁中的分布特征及表达相关因素,并探讨其意义。方法:肝包虫动物模型的制作、HE染色观察、免疫组化观察53例小鼠肝细粒棘球蚴外囊壁中OPN及巨噬细胞的表达与分布。结果:HE染色外囊的着色明显较周围巨噬细胞带深,不存在渐变的过程;83%肝细粒棘球蚴外囊壁中有不同程度OPN表达,且集中分布于肝包虫纤维囊壁(外囊),与外侧巨噬细胞带比较有显著差异(P<0.01)。结论:在动物模型上证明了外囊与肝实质间存在可分离间隙,OPN主要分布在肝细粒棘球蚴外囊,并在其形成过程中起重要作用。更多还原

【Abstract】 Objective: To observe the hydatid cyst in the animal model and to investigate the expression and distribution of osteopontin (OPN) in hydatid cyst and their significances.Methods: 53 mice were infected echinococcus granulosus as to establish the animal model in which the excised cysts were studied with HE and immunohistochemistry staining by using antibodies specific for OPN.Results: It’s obviously disparity between hydatid cyst and macrophages in HE.OPN was selectively distributed in the hydatid cyst while it was approximately not found in macrophages.Conclusion Animal model shows that there is a partible interspace between hydatid cyst and liver parenchyma.OPN plays a important role in hydatid cyst’s formation mechanism.更多还原