【摘要】 研究不同培养条件对梅林青霉液体发酵产纤维素酶的影响,为此对不同碳源、氮源、pH、培养温度、摇瓶转速和摇瓶装量、发酵时间培养条件下羧甲基纤维素酶、滤纸酶、β-葡萄糖苷酶活力进行了测定。结果表明,碳源麦麸诱导效果好于稻草、玉米秸、羊草;氮源中尿素明显降低β-葡萄糖苷酶活力,豆饼、硝酸钠和蛋白胨之间影响不明显;28℃和pH5.0是产纤维素酶最佳温度与酸碱度;通过响应面与等值线分析可知,在摇床转速170~180 r.m in-1,摇瓶装量30~40 mL时,羧甲基纤维素酶、β-葡萄糖苷酶显示较高活性;摇床转速140~150 r.m in-1,摇瓶装量40~50 mL时,滤纸酶显示较高活性;梅林青霉在Bioengineering的NLF19生物反应器液体发酵大约11 d时3种酶活力达到最高。更多还原

【Abstract】 Effects of cultural conditions on cellulase production of Penicillium meilinii in liquid state fermentation were investigated.Activities of CMCase,FPA,BG were determined under the tested conditions of carbon,nitrogen resource,pH,temperature,rotator rate,load,and fermentation time.The results showed that the wheat bran in induction cellulase was better than that of straw,maize stalk and leymus chinensis,the cellulase activity were not evidently affected by the nitrogen resources except urea depressed significantly BG.28 ℃ and pH5.0 were found to be the best optimal fermenting temperatures and acidity,while CMCase and BG showed higher activity in rotator rate of 170～180 r·min-1 and load 30～40 mL,whereas the FPA in the rotator rate of 140～150 r·min-1 and load of 40～50 mL.The cellulase attained activity highest value when the culture time reached to about 11 days using Switzerland bioengineering co NLF19 bioreactor.更多还原