【摘要】 选取包埋剂海藻酸钠浓度为2.0%、3.0%、3.5%、4.0%,固定剂氯化钙浓度为2.0%、3.0%、4.0%、5.0%以及酶液与海藻酸钠溶液包埋比为50%、20%、10%、5%,设计正交实验,将从细菌HB-5中提取到的莠去津降解酶进行固定化,测定不同配比制成的固定化酶对莠去津的降解特性。结果表明,莠去津降解酶的最佳固定化条件为海藻酸钠浓度为3.0%,酶液与海藻酸钠的包埋比为5%,氯化钙浓度为4.0%。最佳固定化时间为4h,适宜的保存温度为4℃。更多还原

【Abstract】 The immobilized methods of free enzyme were studied. The free enzyme extracted from the strain HB-5 was first immobilized according to the orthogonal design,a L9(43) orthogonal array ,whose three factors were sodium alginate,calcium chloride and proportion of sodium alginate and crude enzyme. The design’s three levels were sodium alginate content of 2.0%,3.0%,3.5%,4.0%,respectively,calcium chloride content of 2.0%,3.0%,4.0%,5.0%,respectively and proportion of sodium alginate and crude enzyme of 50%,20%,10%,5%. Subsequently the degradation of immobilized enzyme to degrade atrazine was detected. The results indicated that the free enzyme can be immobilized best under conditions of sodium alginate content of 3.0%,calcium chloride content of 4.0% and proportion of sodium alginate and crude enzyme of 5% for 4 h . Otherwise,the optimum conserved temperature was 4 ℃.更多还原