【摘要】 上颚腺是蜜蜂重要的外分泌腺,其分泌物在蜂群生活中有重要调控作用。为研究中蜂、意蜂三型蜂上颚腺蛋白的异同,对中蜂、意蜂的外勤工蜂、初出房蜂王、巢内雄蜂的上颚腺蛋白进行定量和SDS-PAGE。结果表明:每个腺体中,蜂王的蛋白含量最高,工蜂次之,雄蜂最少,三者差异明显;两蜂种三型蜂的上颚腺蛋白相同的较多,但也有明显差异。意蜂王的76kD蛋白和中蜂王的74k蛋白;意蜂工蜂的54kD蛋白和中蜂工蜂的36kD蛋白;意蜂雄蜂的27kD蛋白和中蜂雄蜂的48kD蛋白;均为相同级型之间的差异蛋白,它们可能与生理功能差异相关。更多还原

【Abstract】 Mandibular gland secretion plays an important role in mediating colonial life. In order to investigate the protein differences of mandibular glands between three castes of Apis cerana cerana and A. mellifera ligustica, the proteins extracted from mandibular glands of newly hatched queens, forager workers, and drones of both species, were quantified respectively and applied for SDS-PAGE. The result showed that, the protein content in the queen’s mandibular gland was highest, the second was the forager, the least the drone in both species. Many protein bands in SDS-PAGE profiles of different castes of both species were the same, though few proteins were evidently different. As for Italian honeybee, a 76kD protein of queen’s mandibular gland, a 54kD protein of forager’s, and a 27kD protein of drone’s were different to those counterparts of Chinese honeybee, which were 74, 36 and 48kD respectively. These different proteins probably reflect the species discrepancy in gland metabolism.更多还原